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cldn1  (Bioss)


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    Structured Review

    Bioss cldn1
    Cldn1, supplied by Bioss, used in various techniques. Bioz Stars score: 92/100, based on 11 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/claudin+1+polyclonal+antibody/Claudin+1+Polyclonal+Antibody/pm41893659-112-10-14
    Average 92 stars, based on 11 article reviews
    cldn1 - by Bioz Stars, 2026-10
    92/100 stars

    Images

    Related Articles

    Recombinant:

    Article Title: Genkwanin reduces airway epithelial cell ferroptosis and alleviates asthma symptoms in mice
    Article Snippet: e buffer. Specific primary antibodies against SAM pointed domain-containing Ets transcription factor (SPDEF, 1:200, 11467-1-AP, ProteinTech Group, Chicago, IL, USA), Claudin-1 (CLDN1, 1:200, bs-1428R, Bioss Biotech, Beijing, China), and autophagy protein 5 (ATG5, 1:100, ab108327, Abcam, Cambridge, MA, USA) were applied overnight at 4 °C. The sections were incubated with goat anti-rabbit IgG antibod

    Western Blot:

    Article Title: Genkwanin reduces airway epithelial cell ferroptosis and alleviates asthma symptoms in mice
    Article Snippet: e buffer. Specific primary antibodies against SAM pointed domain-containing Ets transcription factor (SPDEF, 1:200, 11467-1-AP, ProteinTech Group, Chicago, IL, USA), Claudin-1 (CLDN1, 1:200, bs-1428R, Bioss Biotech, Beijing, China), and autophagy protein 5 (ATG5, 1:100, ab108327, Abcam, Cambridge, MA, USA) were applied overnight at 4 °C. The sections were incubated with goat anti-rabbit IgG antibod

    Immunohistochemistry:

    Article Title: Genkwanin reduces airway epithelial cell ferroptosis and alleviates asthma symptoms in mice
    Article Snippet: e buffer. Specific primary antibodies against SAM pointed domain-containing Ets transcription factor (SPDEF, 1:200, 11467-1-AP, ProteinTech Group, Chicago, IL, USA), Claudin-1 (CLDN1, 1:200, bs-1428R, Bioss Biotech, Beijing, China), and autophagy protein 5 (ATG5, 1:100, ab108327, Abcam, Cambridge, MA, USA) were applied overnight at 4 °C. The sections were incubated with goat anti-rabbit IgG antibod

    Milk:

    Article Title: Genkwanin reduces airway epithelial cell ferroptosis and alleviates asthma symptoms in mice
    Article Snippet: e buffer. Specific primary antibodies against SAM pointed domain-containing Ets transcription factor (SPDEF, 1:200, 11467-1-AP, ProteinTech Group, Chicago, IL, USA), Claudin-1 (CLDN1, 1:200, bs-1428R, Bioss Biotech, Beijing, China), and autophagy protein 5 (ATG5, 1:100, ab108327, Abcam, Cambridge, MA, USA) were applied overnight at 4 °C. The sections were incubated with goat anti-rabbit IgG antibod

    Membrane:

    Article Title: Genkwanin reduces airway epithelial cell ferroptosis and alleviates asthma symptoms in mice
    Article Snippet: e buffer. Specific primary antibodies against SAM pointed domain-containing Ets transcription factor (SPDEF, 1:200, 11467-1-AP, ProteinTech Group, Chicago, IL, USA), Claudin-1 (CLDN1, 1:200, bs-1428R, Bioss Biotech, Beijing, China), and autophagy protein 5 (ATG5, 1:100, ab108327, Abcam, Cambridge, MA, USA) were applied overnight at 4 °C. The sections were incubated with goat anti-rabbit IgG antibod

    Transmission Electron Microscopy:

    Article Title: Genkwanin reduces airway epithelial cell ferroptosis and alleviates asthma symptoms in mice
    Article Snippet: e buffer. Specific primary antibodies against SAM pointed domain-containing Ets transcription factor (SPDEF, 1:200, 11467-1-AP, ProteinTech Group, Chicago, IL, USA), Claudin-1 (CLDN1, 1:200, bs-1428R, Bioss Biotech, Beijing, China), and autophagy protein 5 (ATG5, 1:100, ab108327, Abcam, Cambridge, MA, USA) were applied overnight at 4 °C. The sections were incubated with goat anti-rabbit IgG antibod

    Incubation:

    Article Title: Genkwanin reduces airway epithelial cell ferroptosis and alleviates asthma symptoms in mice
    Article Snippet: e buffer. Specific primary antibodies against SAM pointed domain-containing Ets transcription factor (SPDEF, 1:200, 11467-1-AP, ProteinTech Group, Chicago, IL, USA), Claudin-1 (CLDN1, 1:200, bs-1428R, Bioss Biotech, Beijing, China), and autophagy protein 5 (ATG5, 1:100, ab108327, Abcam, Cambridge, MA, USA) were applied overnight at 4 °C. The sections were incubated with goat anti-rabbit IgG antibod

    Electrophoresis:

    Article Title: Genkwanin reduces airway epithelial cell ferroptosis and alleviates asthma symptoms in mice
    Article Snippet: e buffer. Specific primary antibodies against SAM pointed domain-containing Ets transcription factor (SPDEF, 1:200, 11467-1-AP, ProteinTech Group, Chicago, IL, USA), Claudin-1 (CLDN1, 1:200, bs-1428R, Bioss Biotech, Beijing, China), and autophagy protein 5 (ATG5, 1:100, ab108327, Abcam, Cambridge, MA, USA) were applied overnight at 4 °C. The sections were incubated with goat anti-rabbit IgG antibod

    Polyacrylamide Gel Electrophoresis:

    Article Title: Genkwanin reduces airway epithelial cell ferroptosis and alleviates asthma symptoms in mice
    Article Snippet: e buffer. Specific primary antibodies against SAM pointed domain-containing Ets transcription factor (SPDEF, 1:200, 11467-1-AP, ProteinTech Group, Chicago, IL, USA), Claudin-1 (CLDN1, 1:200, bs-1428R, Bioss Biotech, Beijing, China), and autophagy protein 5 (ATG5, 1:100, ab108327, Abcam, Cambridge, MA, USA) were applied overnight at 4 °C. The sections were incubated with goat anti-rabbit IgG antibod

    SDS Page:

    Article Title: Genkwanin reduces airway epithelial cell ferroptosis and alleviates asthma symptoms in mice
    Article Snippet: e buffer. Specific primary antibodies against SAM pointed domain-containing Ets transcription factor (SPDEF, 1:200, 11467-1-AP, ProteinTech Group, Chicago, IL, USA), Claudin-1 (CLDN1, 1:200, bs-1428R, Bioss Biotech, Beijing, China), and autophagy protein 5 (ATG5, 1:100, ab108327, Abcam, Cambridge, MA, USA) were applied overnight at 4 °C. The sections were incubated with goat anti-rabbit IgG antibod

    Isolation:

    Article Title: Genkwanin reduces airway epithelial cell ferroptosis and alleviates asthma symptoms in mice
    Article Snippet: e buffer. Specific primary antibodies against SAM pointed domain-containing Ets transcription factor (SPDEF, 1:200, 11467-1-AP, ProteinTech Group, Chicago, IL, USA), Claudin-1 (CLDN1, 1:200, bs-1428R, Bioss Biotech, Beijing, China), and autophagy protein 5 (ATG5, 1:100, ab108327, Abcam, Cambridge, MA, USA) were applied overnight at 4 °C. The sections were incubated with goat anti-rabbit IgG antibod

    Nucleic Acid Electrophoresis:

    Article Title: Genkwanin reduces airway epithelial cell ferroptosis and alleviates asthma symptoms in mice
    Article Snippet: e buffer. Specific primary antibodies against SAM pointed domain-containing Ets transcription factor (SPDEF, 1:200, 11467-1-AP, ProteinTech Group, Chicago, IL, USA), Claudin-1 (CLDN1, 1:200, bs-1428R, Bioss Biotech, Beijing, China), and autophagy protein 5 (ATG5, 1:100, ab108327, Abcam, Cambridge, MA, USA) were applied overnight at 4 °C. The sections were incubated with goat anti-rabbit IgG antibod



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    The mRNA expression levels of mucin and TJ proteins in each treatment group after the addition of MAPK activator to Zonulin overexpressing cell lines. ( A ) Relative expression of MUC1 mRNA; ( B ) Relative expression of MUC2 mRNA; ( C ) Relative expression of ZO-1 mRNA; ( D ) Relative mRNA expression of Occludin; ( E ) Relative expression of <t>Claudin</t> <t>1</t> mRNA; ( F ) Relative expression of Zonulin mRNA. * p < 0.05, ** p < 0.01.
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    The mRNA expression levels of mucin and TJ proteins in each treatment group after the addition of MAPK activator to Zonulin overexpressing cell lines. ( A ) Relative expression of MUC1 mRNA; ( B ) Relative expression of MUC2 mRNA; ( C ) Relative expression of ZO-1 mRNA; ( D ) Relative mRNA expression of Occludin; ( E ) Relative expression of <t>Claudin</t> <t>1</t> mRNA; ( F ) Relative expression of Zonulin mRNA. * p < 0.05, ** p < 0.01.
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    The mRNA expression levels of mucin and TJ proteins in each treatment group after the addition of MAPK activator to Zonulin overexpressing cell lines. ( A ) Relative expression of MUC1 mRNA; ( B ) Relative expression of MUC2 mRNA; ( C ) Relative expression of ZO-1 mRNA; ( D ) Relative mRNA expression of Occludin; ( E ) Relative expression of <t>Claudin</t> <t>1</t> mRNA; ( F ) Relative expression of Zonulin mRNA. * p < 0.05, ** p < 0.01.
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    NaB reduced bacterial translocation and LPS levels by enhancing the barrier function of the colonic and cardiac tissues (A) Histopathological damage of colonic tissue in the DOX+NaB, DOX +NaCl group by Hematoxylin and Eosin (H&E) staining (scale bars, 50 μm). (B and C) Representative images, quantitative analysis <t>of</t> <t>Claudin-1</t> (B) and ZO-1 (C) of colonic tissue in the DOX+NaB, DOX+NaCl group by immunofluorescence (scale bars, 50 μm). (D) Representative images of goblet cell changes of colonic tissue in the DOX+NaB, DOX+NaCl group by AB-PAS staining (scale bars, 50 μm). (E) The mRNA expression levels of Muc2 and Tff3 in the colonic tissue via qRT-PCR assay in the DOX+NaB and DOX+NaCl groups. (F) Serum and cardiac tissue LPS concentrations in the DOX+NaB and DOX+NaCl groups via ELISA. (G and H) Representative images (G), quantitative analysis (H) of EUB338 bacterial probe count in cardiac tissue of the DOX+NaB and DOX+NaCl groups by FISH (scale bars, 20 μm). (I) The Pearson’s correlation analysis of EUB338 probe count and LVEF decrease rate in the DOX+NaB and DOX+NaCl groups. Statistical significance was determined using the simple linear regression of Correlation. (J) Representative images and quantitative analysis of connexin-43 and N-cadherin in cardiac tissue of the DOX+ NaB, DOX + NaCl group by immunofluorescence (scale bars, 50 μm). DOX+NaB and DOX+NaCl groups, n = 8 per group. Error bars represent the mean ± SEM. For normally distributed data with homogeneous variances, statistical analyses were performed using Student’s t tests for two groups and one-way ANOVA for multiple comparisons. ∗ p < 0.05; ∗∗ p < 0.01; ∗∗∗ p < 0.001; ∗∗∗∗ p < 0.0001.
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    Image Search Results


    The mRNA expression levels of mucin and TJ proteins in each treatment group after the addition of MAPK activator to Zonulin overexpressing cell lines. ( A ) Relative expression of MUC1 mRNA; ( B ) Relative expression of MUC2 mRNA; ( C ) Relative expression of ZO-1 mRNA; ( D ) Relative mRNA expression of Occludin; ( E ) Relative expression of Claudin 1 mRNA; ( F ) Relative expression of Zonulin mRNA. * p < 0.05, ** p < 0.01.

    Journal: Scientific Reports

    Article Title: Lactobacillus yoelii Lac-2 mediates the mechanism of MAPK signaling pathway involved in the regulation of intestinal barrier and pathogen translocation by Zonulin expression

    doi: 10.1038/s41598-026-55430-y

    Figure Lengend Snippet: The mRNA expression levels of mucin and TJ proteins in each treatment group after the addition of MAPK activator to Zonulin overexpressing cell lines. ( A ) Relative expression of MUC1 mRNA; ( B ) Relative expression of MUC2 mRNA; ( C ) Relative expression of ZO-1 mRNA; ( D ) Relative mRNA expression of Occludin; ( E ) Relative expression of Claudin 1 mRNA; ( F ) Relative expression of Zonulin mRNA. * p < 0.05, ** p < 0.01.

    Article Snippet: Primary antibodies included p38 rabbit polyclonal antibody (A0027, Abclonal, Wuhan, China), ERK rabbit polyclonal antibody (AP1120, Abclonal, Wuhan, China), JNK rabbit polyclonal antibody (A18678, Abclonal, Wuhan, China), zonulin rabbit polyclonal antibody (A1571, Abclonal, Wuhan, China), β-Actin rabbit monoclonal antibody (highly sensitive) (AC026, Abclonal, Wuhan, China), MUC1 rabbit polyclonal antibody (A21726, Abclonal, Wuhan, China), MUC2 rabbit polyclonal antibody (A14659, Abclonal, Wuhan, China), ZO-1 rabbit polyclonal antibody (A0659, Abclonal, Wuhan, China), occludin rabbit polyclonal antibody (A12621, Abclonal, Wuhan, China), claudin 1 rabbit polyclonal antibody (A21770, Abclonal, Wuhan, China), and HRP-conjugated sheep anti-rabbit IgG (H + L) (A0208, Abclonal, Wuhan, China).

    Techniques: Expressing

    The mRNA expression levels of mucin and TJ proteins in each treatment group after the addition of MAPK blocker to the Zonulin up-regulation cell line. ( A ) Relative expression of MUC1 mRNA; ( B ) Relative expression of MUC2 mRNA; ( C ) Relative expression of ZO-1 mRNA; ( D ) Relative mRNA expression of Occludin; ( E ) Relative expression of Claudin 1 mRNA; ( F ) Relative expression of Zonulin mRNA. * p < 0.05, ** p < 0.01.

    Journal: Scientific Reports

    Article Title: Lactobacillus yoelii Lac-2 mediates the mechanism of MAPK signaling pathway involved in the regulation of intestinal barrier and pathogen translocation by Zonulin expression

    doi: 10.1038/s41598-026-55430-y

    Figure Lengend Snippet: The mRNA expression levels of mucin and TJ proteins in each treatment group after the addition of MAPK blocker to the Zonulin up-regulation cell line. ( A ) Relative expression of MUC1 mRNA; ( B ) Relative expression of MUC2 mRNA; ( C ) Relative expression of ZO-1 mRNA; ( D ) Relative mRNA expression of Occludin; ( E ) Relative expression of Claudin 1 mRNA; ( F ) Relative expression of Zonulin mRNA. * p < 0.05, ** p < 0.01.

    Article Snippet: Primary antibodies included p38 rabbit polyclonal antibody (A0027, Abclonal, Wuhan, China), ERK rabbit polyclonal antibody (AP1120, Abclonal, Wuhan, China), JNK rabbit polyclonal antibody (A18678, Abclonal, Wuhan, China), zonulin rabbit polyclonal antibody (A1571, Abclonal, Wuhan, China), β-Actin rabbit monoclonal antibody (highly sensitive) (AC026, Abclonal, Wuhan, China), MUC1 rabbit polyclonal antibody (A21726, Abclonal, Wuhan, China), MUC2 rabbit polyclonal antibody (A14659, Abclonal, Wuhan, China), ZO-1 rabbit polyclonal antibody (A0659, Abclonal, Wuhan, China), occludin rabbit polyclonal antibody (A12621, Abclonal, Wuhan, China), claudin 1 rabbit polyclonal antibody (A21770, Abclonal, Wuhan, China), and HRP-conjugated sheep anti-rabbit IgG (H + L) (A0208, Abclonal, Wuhan, China).

    Techniques: Expressing

    The mRNA expression levels of mucin and TJ protein in each treatment group were reduced by Zonulin knockdown cell line after MAPK activator was added. ( A ) Relative expression of MUC1 mRNA; ( B ) Relative expression of MUC2 mRNA; ( C ) Relative expression of ZO-1 mRNA; ( D ) Relative mRNA expression of Occludin; ( E ) Relative expression of Claudin 1 mRNA; ( F ) Relative expression of Zonulin mRNA. * p < 0.05, ** p < 0.01.

    Journal: Scientific Reports

    Article Title: Lactobacillus yoelii Lac-2 mediates the mechanism of MAPK signaling pathway involved in the regulation of intestinal barrier and pathogen translocation by Zonulin expression

    doi: 10.1038/s41598-026-55430-y

    Figure Lengend Snippet: The mRNA expression levels of mucin and TJ protein in each treatment group were reduced by Zonulin knockdown cell line after MAPK activator was added. ( A ) Relative expression of MUC1 mRNA; ( B ) Relative expression of MUC2 mRNA; ( C ) Relative expression of ZO-1 mRNA; ( D ) Relative mRNA expression of Occludin; ( E ) Relative expression of Claudin 1 mRNA; ( F ) Relative expression of Zonulin mRNA. * p < 0.05, ** p < 0.01.

    Article Snippet: Primary antibodies included p38 rabbit polyclonal antibody (A0027, Abclonal, Wuhan, China), ERK rabbit polyclonal antibody (AP1120, Abclonal, Wuhan, China), JNK rabbit polyclonal antibody (A18678, Abclonal, Wuhan, China), zonulin rabbit polyclonal antibody (A1571, Abclonal, Wuhan, China), β-Actin rabbit monoclonal antibody (highly sensitive) (AC026, Abclonal, Wuhan, China), MUC1 rabbit polyclonal antibody (A21726, Abclonal, Wuhan, China), MUC2 rabbit polyclonal antibody (A14659, Abclonal, Wuhan, China), ZO-1 rabbit polyclonal antibody (A0659, Abclonal, Wuhan, China), occludin rabbit polyclonal antibody (A12621, Abclonal, Wuhan, China), claudin 1 rabbit polyclonal antibody (A21770, Abclonal, Wuhan, China), and HRP-conjugated sheep anti-rabbit IgG (H + L) (A0208, Abclonal, Wuhan, China).

    Techniques: Expressing, Knockdown

    The mRNA expression levels of mucin and TJ proteins in each treatment group after Zonulin knockdown cell line was supplemented with MAPK blocker. ( A ) Relative expression of MUC1 mRNA; ( B ) Relative expression of MUC2 mRNA; ( C ) Relative expression of ZO-1 mRNA; ( D ) Relative mRNA expression of Occludin; ( E ) Relative expression of Claudin 1 mRNA; ( F ) Relative expression of Zonulin mRNA. * p < 0.05, ** p < 0.01.

    Journal: Scientific Reports

    Article Title: Lactobacillus yoelii Lac-2 mediates the mechanism of MAPK signaling pathway involved in the regulation of intestinal barrier and pathogen translocation by Zonulin expression

    doi: 10.1038/s41598-026-55430-y

    Figure Lengend Snippet: The mRNA expression levels of mucin and TJ proteins in each treatment group after Zonulin knockdown cell line was supplemented with MAPK blocker. ( A ) Relative expression of MUC1 mRNA; ( B ) Relative expression of MUC2 mRNA; ( C ) Relative expression of ZO-1 mRNA; ( D ) Relative mRNA expression of Occludin; ( E ) Relative expression of Claudin 1 mRNA; ( F ) Relative expression of Zonulin mRNA. * p < 0.05, ** p < 0.01.

    Article Snippet: Primary antibodies included p38 rabbit polyclonal antibody (A0027, Abclonal, Wuhan, China), ERK rabbit polyclonal antibody (AP1120, Abclonal, Wuhan, China), JNK rabbit polyclonal antibody (A18678, Abclonal, Wuhan, China), zonulin rabbit polyclonal antibody (A1571, Abclonal, Wuhan, China), β-Actin rabbit monoclonal antibody (highly sensitive) (AC026, Abclonal, Wuhan, China), MUC1 rabbit polyclonal antibody (A21726, Abclonal, Wuhan, China), MUC2 rabbit polyclonal antibody (A14659, Abclonal, Wuhan, China), ZO-1 rabbit polyclonal antibody (A0659, Abclonal, Wuhan, China), occludin rabbit polyclonal antibody (A12621, Abclonal, Wuhan, China), claudin 1 rabbit polyclonal antibody (A21770, Abclonal, Wuhan, China), and HRP-conjugated sheep anti-rabbit IgG (H + L) (A0208, Abclonal, Wuhan, China).

    Techniques: Expressing, Knockdown

    Zonulin up-regulation and Zonulin knockdown cell lines were treated with MAPK activator and MAPK blocker mRNA expression levels. ( A ) Relative expression of MUC1 mRNA; ( B ) Relative expression of MUC2 mRNA; ( C ) Relative expression of ZO-1 mRNA; ( D ) Relative mRNA expression of Occludin; ( E ) Relative expression of Claudin 1 mRNA; ( F ) Relative expression of Zonulin mRNA. * p < 0.05, ** p < 0.01.

    Journal: Scientific Reports

    Article Title: Lactobacillus yoelii Lac-2 mediates the mechanism of MAPK signaling pathway involved in the regulation of intestinal barrier and pathogen translocation by Zonulin expression

    doi: 10.1038/s41598-026-55430-y

    Figure Lengend Snippet: Zonulin up-regulation and Zonulin knockdown cell lines were treated with MAPK activator and MAPK blocker mRNA expression levels. ( A ) Relative expression of MUC1 mRNA; ( B ) Relative expression of MUC2 mRNA; ( C ) Relative expression of ZO-1 mRNA; ( D ) Relative mRNA expression of Occludin; ( E ) Relative expression of Claudin 1 mRNA; ( F ) Relative expression of Zonulin mRNA. * p < 0.05, ** p < 0.01.

    Article Snippet: Primary antibodies included p38 rabbit polyclonal antibody (A0027, Abclonal, Wuhan, China), ERK rabbit polyclonal antibody (AP1120, Abclonal, Wuhan, China), JNK rabbit polyclonal antibody (A18678, Abclonal, Wuhan, China), zonulin rabbit polyclonal antibody (A1571, Abclonal, Wuhan, China), β-Actin rabbit monoclonal antibody (highly sensitive) (AC026, Abclonal, Wuhan, China), MUC1 rabbit polyclonal antibody (A21726, Abclonal, Wuhan, China), MUC2 rabbit polyclonal antibody (A14659, Abclonal, Wuhan, China), ZO-1 rabbit polyclonal antibody (A0659, Abclonal, Wuhan, China), occludin rabbit polyclonal antibody (A12621, Abclonal, Wuhan, China), claudin 1 rabbit polyclonal antibody (A21770, Abclonal, Wuhan, China), and HRP-conjugated sheep anti-rabbit IgG (H + L) (A0208, Abclonal, Wuhan, China).

    Techniques: Knockdown, Expressing

    The expression levels of mucin and TJ protein in each treatment group after the addition of MAPK activator to Zonulin up-regulation cell line. ( A ) Gray value detection results of mucin WB; ( B ) Gray value of TJ protein WB; ( C ) Relative β-actin expression level of MUC1; ( D ) Relative β-actin expression level of MUC2; ( E ) Relative β-actin expression level of ZO-1; ( F ) Relative β-actin expression level of Occludin; ( G ) Claudin 1 relative β-actin expression level; ( H ) Relative β-actin expression levels of Zonulin. * p < 0.05, ** p < 0.01.

    Journal: Scientific Reports

    Article Title: Lactobacillus yoelii Lac-2 mediates the mechanism of MAPK signaling pathway involved in the regulation of intestinal barrier and pathogen translocation by Zonulin expression

    doi: 10.1038/s41598-026-55430-y

    Figure Lengend Snippet: The expression levels of mucin and TJ protein in each treatment group after the addition of MAPK activator to Zonulin up-regulation cell line. ( A ) Gray value detection results of mucin WB; ( B ) Gray value of TJ protein WB; ( C ) Relative β-actin expression level of MUC1; ( D ) Relative β-actin expression level of MUC2; ( E ) Relative β-actin expression level of ZO-1; ( F ) Relative β-actin expression level of Occludin; ( G ) Claudin 1 relative β-actin expression level; ( H ) Relative β-actin expression levels of Zonulin. * p < 0.05, ** p < 0.01.

    Article Snippet: Primary antibodies included p38 rabbit polyclonal antibody (A0027, Abclonal, Wuhan, China), ERK rabbit polyclonal antibody (AP1120, Abclonal, Wuhan, China), JNK rabbit polyclonal antibody (A18678, Abclonal, Wuhan, China), zonulin rabbit polyclonal antibody (A1571, Abclonal, Wuhan, China), β-Actin rabbit monoclonal antibody (highly sensitive) (AC026, Abclonal, Wuhan, China), MUC1 rabbit polyclonal antibody (A21726, Abclonal, Wuhan, China), MUC2 rabbit polyclonal antibody (A14659, Abclonal, Wuhan, China), ZO-1 rabbit polyclonal antibody (A0659, Abclonal, Wuhan, China), occludin rabbit polyclonal antibody (A12621, Abclonal, Wuhan, China), claudin 1 rabbit polyclonal antibody (A21770, Abclonal, Wuhan, China), and HRP-conjugated sheep anti-rabbit IgG (H + L) (A0208, Abclonal, Wuhan, China).

    Techniques: Expressing

    Expression levels of mucin and TJ protein in each treatment group after the addition of MAPK blocker to Zonulin up-regulation cell line. ( A ) Gray value detection results of mucin WB; ( B ) Gray value of TJ protein WB; ( C ) Relative β-actin expression level of MUC1; ( D ) Relative β-actin expression level of MUC2; ( E ) Relative β-actin expression level of ZO-1; ( F ) Relative β-actin expression level of Occludin; ( G ) Claudin 1 relative β-actin expression level; ( H ) Relative β-actin expression levels of Zonulin. * p < 0.05, ** p < 0.01.

    Journal: Scientific Reports

    Article Title: Lactobacillus yoelii Lac-2 mediates the mechanism of MAPK signaling pathway involved in the regulation of intestinal barrier and pathogen translocation by Zonulin expression

    doi: 10.1038/s41598-026-55430-y

    Figure Lengend Snippet: Expression levels of mucin and TJ protein in each treatment group after the addition of MAPK blocker to Zonulin up-regulation cell line. ( A ) Gray value detection results of mucin WB; ( B ) Gray value of TJ protein WB; ( C ) Relative β-actin expression level of MUC1; ( D ) Relative β-actin expression level of MUC2; ( E ) Relative β-actin expression level of ZO-1; ( F ) Relative β-actin expression level of Occludin; ( G ) Claudin 1 relative β-actin expression level; ( H ) Relative β-actin expression levels of Zonulin. * p < 0.05, ** p < 0.01.

    Article Snippet: Primary antibodies included p38 rabbit polyclonal antibody (A0027, Abclonal, Wuhan, China), ERK rabbit polyclonal antibody (AP1120, Abclonal, Wuhan, China), JNK rabbit polyclonal antibody (A18678, Abclonal, Wuhan, China), zonulin rabbit polyclonal antibody (A1571, Abclonal, Wuhan, China), β-Actin rabbit monoclonal antibody (highly sensitive) (AC026, Abclonal, Wuhan, China), MUC1 rabbit polyclonal antibody (A21726, Abclonal, Wuhan, China), MUC2 rabbit polyclonal antibody (A14659, Abclonal, Wuhan, China), ZO-1 rabbit polyclonal antibody (A0659, Abclonal, Wuhan, China), occludin rabbit polyclonal antibody (A12621, Abclonal, Wuhan, China), claudin 1 rabbit polyclonal antibody (A21770, Abclonal, Wuhan, China), and HRP-conjugated sheep anti-rabbit IgG (H + L) (A0208, Abclonal, Wuhan, China).

    Techniques: Expressing

    The expression levels of mucin and TJ protein in each treatment group after Zonulin knockdown cell line was supplemented with MAPK activator. ( A ) Gray value detection results of mucin WB; ( B ) Gray value of TJ protein WB; ( C ) Relative β-actin expression level of MUC1; ( D ) Relative β-actin expression level of MUC2; ( E ) Relative β-actin expression level of ZO-1; ( F ) Relative β-actin expression level of Occludin; ( G ) Claudin 1 relative β-actin expression level; ( H ) Relative β-actin expression levels of Zonulin. * p < 0.05, ** p < 0.01.

    Journal: Scientific Reports

    Article Title: Lactobacillus yoelii Lac-2 mediates the mechanism of MAPK signaling pathway involved in the regulation of intestinal barrier and pathogen translocation by Zonulin expression

    doi: 10.1038/s41598-026-55430-y

    Figure Lengend Snippet: The expression levels of mucin and TJ protein in each treatment group after Zonulin knockdown cell line was supplemented with MAPK activator. ( A ) Gray value detection results of mucin WB; ( B ) Gray value of TJ protein WB; ( C ) Relative β-actin expression level of MUC1; ( D ) Relative β-actin expression level of MUC2; ( E ) Relative β-actin expression level of ZO-1; ( F ) Relative β-actin expression level of Occludin; ( G ) Claudin 1 relative β-actin expression level; ( H ) Relative β-actin expression levels of Zonulin. * p < 0.05, ** p < 0.01.

    Article Snippet: Primary antibodies included p38 rabbit polyclonal antibody (A0027, Abclonal, Wuhan, China), ERK rabbit polyclonal antibody (AP1120, Abclonal, Wuhan, China), JNK rabbit polyclonal antibody (A18678, Abclonal, Wuhan, China), zonulin rabbit polyclonal antibody (A1571, Abclonal, Wuhan, China), β-Actin rabbit monoclonal antibody (highly sensitive) (AC026, Abclonal, Wuhan, China), MUC1 rabbit polyclonal antibody (A21726, Abclonal, Wuhan, China), MUC2 rabbit polyclonal antibody (A14659, Abclonal, Wuhan, China), ZO-1 rabbit polyclonal antibody (A0659, Abclonal, Wuhan, China), occludin rabbit polyclonal antibody (A12621, Abclonal, Wuhan, China), claudin 1 rabbit polyclonal antibody (A21770, Abclonal, Wuhan, China), and HRP-conjugated sheep anti-rabbit IgG (H + L) (A0208, Abclonal, Wuhan, China).

    Techniques: Expressing, Knockdown

    The expression levels of mucin and TJ protein in each treatment group after Zonulin knockdown cell line was supplemented with MAPK blocker. ( A ) Gray value detection results of mucin WB; ( B ) Gray value of TJ protein WB; ( C ) Relative β-actin expression level of MUC1; ( D ) Relative β-actin expression level of MUC2; ( E ) Relative β-actin expression level of ZO-1; ( F ) Relative β-actin expression level of Occludin; ( G ) Claudin 1 relative β-actin expression level; ( H ) Relative β-actin expression levels of Zonulin. * p < 0.05, ** p < 0.01.

    Journal: Scientific Reports

    Article Title: Lactobacillus yoelii Lac-2 mediates the mechanism of MAPK signaling pathway involved in the regulation of intestinal barrier and pathogen translocation by Zonulin expression

    doi: 10.1038/s41598-026-55430-y

    Figure Lengend Snippet: The expression levels of mucin and TJ protein in each treatment group after Zonulin knockdown cell line was supplemented with MAPK blocker. ( A ) Gray value detection results of mucin WB; ( B ) Gray value of TJ protein WB; ( C ) Relative β-actin expression level of MUC1; ( D ) Relative β-actin expression level of MUC2; ( E ) Relative β-actin expression level of ZO-1; ( F ) Relative β-actin expression level of Occludin; ( G ) Claudin 1 relative β-actin expression level; ( H ) Relative β-actin expression levels of Zonulin. * p < 0.05, ** p < 0.01.

    Article Snippet: Primary antibodies included p38 rabbit polyclonal antibody (A0027, Abclonal, Wuhan, China), ERK rabbit polyclonal antibody (AP1120, Abclonal, Wuhan, China), JNK rabbit polyclonal antibody (A18678, Abclonal, Wuhan, China), zonulin rabbit polyclonal antibody (A1571, Abclonal, Wuhan, China), β-Actin rabbit monoclonal antibody (highly sensitive) (AC026, Abclonal, Wuhan, China), MUC1 rabbit polyclonal antibody (A21726, Abclonal, Wuhan, China), MUC2 rabbit polyclonal antibody (A14659, Abclonal, Wuhan, China), ZO-1 rabbit polyclonal antibody (A0659, Abclonal, Wuhan, China), occludin rabbit polyclonal antibody (A12621, Abclonal, Wuhan, China), claudin 1 rabbit polyclonal antibody (A21770, Abclonal, Wuhan, China), and HRP-conjugated sheep anti-rabbit IgG (H + L) (A0208, Abclonal, Wuhan, China).

    Techniques: Expressing, Knockdown

    Zonulin up-regulation and Zonulin knockdown cell lines were treated with MAPK activator and MAPK blocker. ( A ) Gray value detection results of mucin WB; ( B ) Gray value of TJ protein WB; ( C ) Relative β-actin expression level of MUC1; ( D ) Relative β-actin expression level of MUC2; ( E ) Relative β-actin expression level of ZO-1; ( F ) Relative β-actin expression level of Occludin; ( G ) Claudin 1 relative β-actin expression level; ( H ) Relative β-actin expression levels of Zonulin. * p < 0.05, ** p < 0.01.

    Journal: Scientific Reports

    Article Title: Lactobacillus yoelii Lac-2 mediates the mechanism of MAPK signaling pathway involved in the regulation of intestinal barrier and pathogen translocation by Zonulin expression

    doi: 10.1038/s41598-026-55430-y

    Figure Lengend Snippet: Zonulin up-regulation and Zonulin knockdown cell lines were treated with MAPK activator and MAPK blocker. ( A ) Gray value detection results of mucin WB; ( B ) Gray value of TJ protein WB; ( C ) Relative β-actin expression level of MUC1; ( D ) Relative β-actin expression level of MUC2; ( E ) Relative β-actin expression level of ZO-1; ( F ) Relative β-actin expression level of Occludin; ( G ) Claudin 1 relative β-actin expression level; ( H ) Relative β-actin expression levels of Zonulin. * p < 0.05, ** p < 0.01.

    Article Snippet: Primary antibodies included p38 rabbit polyclonal antibody (A0027, Abclonal, Wuhan, China), ERK rabbit polyclonal antibody (AP1120, Abclonal, Wuhan, China), JNK rabbit polyclonal antibody (A18678, Abclonal, Wuhan, China), zonulin rabbit polyclonal antibody (A1571, Abclonal, Wuhan, China), β-Actin rabbit monoclonal antibody (highly sensitive) (AC026, Abclonal, Wuhan, China), MUC1 rabbit polyclonal antibody (A21726, Abclonal, Wuhan, China), MUC2 rabbit polyclonal antibody (A14659, Abclonal, Wuhan, China), ZO-1 rabbit polyclonal antibody (A0659, Abclonal, Wuhan, China), occludin rabbit polyclonal antibody (A12621, Abclonal, Wuhan, China), claudin 1 rabbit polyclonal antibody (A21770, Abclonal, Wuhan, China), and HRP-conjugated sheep anti-rabbit IgG (H + L) (A0208, Abclonal, Wuhan, China).

    Techniques: Knockdown, Expressing

    NaB reduced bacterial translocation and LPS levels by enhancing the barrier function of the colonic and cardiac tissues (A) Histopathological damage of colonic tissue in the DOX+NaB, DOX +NaCl group by Hematoxylin and Eosin (H&E) staining (scale bars, 50 μm). (B and C) Representative images, quantitative analysis of Claudin-1 (B) and ZO-1 (C) of colonic tissue in the DOX+NaB, DOX+NaCl group by immunofluorescence (scale bars, 50 μm). (D) Representative images of goblet cell changes of colonic tissue in the DOX+NaB, DOX+NaCl group by AB-PAS staining (scale bars, 50 μm). (E) The mRNA expression levels of Muc2 and Tff3 in the colonic tissue via qRT-PCR assay in the DOX+NaB and DOX+NaCl groups. (F) Serum and cardiac tissue LPS concentrations in the DOX+NaB and DOX+NaCl groups via ELISA. (G and H) Representative images (G), quantitative analysis (H) of EUB338 bacterial probe count in cardiac tissue of the DOX+NaB and DOX+NaCl groups by FISH (scale bars, 20 μm). (I) The Pearson’s correlation analysis of EUB338 probe count and LVEF decrease rate in the DOX+NaB and DOX+NaCl groups. Statistical significance was determined using the simple linear regression of Correlation. (J) Representative images and quantitative analysis of connexin-43 and N-cadherin in cardiac tissue of the DOX+ NaB, DOX + NaCl group by immunofluorescence (scale bars, 50 μm). DOX+NaB and DOX+NaCl groups, n = 8 per group. Error bars represent the mean ± SEM. For normally distributed data with homogeneous variances, statistical analyses were performed using Student’s t tests for two groups and one-way ANOVA for multiple comparisons. ∗ p < 0.05; ∗∗ p < 0.01; ∗∗∗ p < 0.001; ∗∗∗∗ p < 0.0001.

    Journal: iScience

    Article Title: Butyrate ameliorates doxorubicin-induced heart failure by inhibiting cardiomyocyte ferroptosis through the gut-heart axis

    doi: 10.1016/j.isci.2026.114754

    Figure Lengend Snippet: NaB reduced bacterial translocation and LPS levels by enhancing the barrier function of the colonic and cardiac tissues (A) Histopathological damage of colonic tissue in the DOX+NaB, DOX +NaCl group by Hematoxylin and Eosin (H&E) staining (scale bars, 50 μm). (B and C) Representative images, quantitative analysis of Claudin-1 (B) and ZO-1 (C) of colonic tissue in the DOX+NaB, DOX+NaCl group by immunofluorescence (scale bars, 50 μm). (D) Representative images of goblet cell changes of colonic tissue in the DOX+NaB, DOX+NaCl group by AB-PAS staining (scale bars, 50 μm). (E) The mRNA expression levels of Muc2 and Tff3 in the colonic tissue via qRT-PCR assay in the DOX+NaB and DOX+NaCl groups. (F) Serum and cardiac tissue LPS concentrations in the DOX+NaB and DOX+NaCl groups via ELISA. (G and H) Representative images (G), quantitative analysis (H) of EUB338 bacterial probe count in cardiac tissue of the DOX+NaB and DOX+NaCl groups by FISH (scale bars, 20 μm). (I) The Pearson’s correlation analysis of EUB338 probe count and LVEF decrease rate in the DOX+NaB and DOX+NaCl groups. Statistical significance was determined using the simple linear regression of Correlation. (J) Representative images and quantitative analysis of connexin-43 and N-cadherin in cardiac tissue of the DOX+ NaB, DOX + NaCl group by immunofluorescence (scale bars, 50 μm). DOX+NaB and DOX+NaCl groups, n = 8 per group. Error bars represent the mean ± SEM. For normally distributed data with homogeneous variances, statistical analyses were performed using Student’s t tests for two groups and one-way ANOVA for multiple comparisons. ∗ p < 0.05; ∗∗ p < 0.01; ∗∗∗ p < 0.001; ∗∗∗∗ p < 0.0001.

    Article Snippet: Rabbit Claudin 1 Polyclonal antibody , proteintech , Cat# 28674-1-AP; RRID: AB_2881190.

    Techniques: Translocation Assay, Staining, Immunofluorescence, Expressing, Quantitative RT-PCR, Enzyme-linked Immunosorbent Assay